Louisville police say there were no injuries, but school children were able to hear the gunshots near downtown.
The Louisville Metro Police Department shared several photos from surveillance video of the suspect in a post on its Facebook page Tuesday.
LMPD said the ambulance was stolen from the bay at Jewish Hospital downtown around 7:30 p.m. Friday, while the crew was inside the hospital with a patient.
LMPD's request for help finding the man comes a day after they asked for help finding a different suspect accused of punching a TARC driver in the face back in August in NuLu.
A camera at the Kentucky International Convention Center in downtown Louisville captured the moments someone opened fire at a nearby restaurant, leaving two people dead and hundreds scrambling for safety.