A company spokesperson said the plant was expected to resume normal operations on Friday.
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It's unknown at this time what the substance was or how much was spilled.
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The spill happened after a train derailed on Feb. 3 in East Palestine, Ohio, near the Pennsylvania border.
On March 11, Louisville Metro emergency management agencies responded to complaints of an odor in the area near a chemical manufacturer.
KSP says the spill has triggered a small evacuation that does not include any homes. The radius is only 800 feet.
A day after a hazardous chemical leak, Dupont workers returned to the job even as hydrochloric acid continues to spill.
Dixie Highway (U.S. 31 W) is expected to remain closed until further notice while crews clean up after Monday's train derailment.