A tree blocked the eastbound lanes Thursday.
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Thousands of people are still without power in Kentucky as clean up efforts continue after severe weather moved through the area on Sunday.
The interstate will be closed for two nights for a routine inspection of the Cochran Hill Tunnels.
A man was hit and killed Saturday afternoon on Interstate 64 near Cannons Lane.
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Interstate 64 is expected to be closed in both directions for the next four weekends because of work on the Cochran Hill Tunnels. But after the first weekend of closures and detours, problems popped up.
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Interstate 64 will be closed this weekend in both directions at the Cochran Hill Tunnels, according to the Kentucky Transportation Cabinet (KYTC).