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A necropsy will be performed and any additional information from the results will be released later.
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It's known as the EEHV virus.
The Louisville Zoo celebrated two of its biggest residents on Saturday.
The zoo will also celebrate the 18th birthday of Gremlin, the California sea lion.
Fitz was the first elephant born at the zoo in decades.
The zoo also plans to launch a “behind-the-scenes sloth experience” that will allow visitors to meet and greet with one of the two Linneaus’s two-toed sloths.
The Louisville Zoo said that its calf, Fitz, was sired by Jackson, a 40-year-old African bull elephant at the Pittsburgh Zoo.