The series of races leads up to the the Kentucky Derby Festival Marathon and miniMarathon, and includes a 5K, a 10K and a 15K.
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The race begins at 8 a.m. in downtown Louisville, kicking off at Louisville Slugger Field.
The series of races will kick off March 2 with a 5K, followed by a 10K on March 16. The series will finish with a 15K on March 30.
Main Street from 2nd and 3rd Streets will be open to traffic before and during the race.
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Louisville Metro Police have released plans for road closures, restricted access and no parking areas for the Triple Crown 10K on Saturday.
The first race of the annual running series, the 5K, starts at 8 a.m. at Slugger Field at Brook and East Market streets in downtown Louisville.
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RAM Racing announced on Wednesday that it has acquired the three races that had been in jeopardy.
Louisville's Triple Crown of Running may not have reached the finish line just yet.
The 3.1 mile race ended on Main Street near Slugger Field, and the top finishers for women and men were both hometown competitors.