The seven-acre Wildlife in Need property owned by Tim Stark is being auctioned on Saturday.
PETA sued Stark in 2017 for violating the Endangered Species Act when it learned the zoo owner paid a vet to declaw big cats for controversial "Tiger Baby Playtime" sessions with visitors.
Animal advocates are claiming victory after a controversial roadside zoo in southern Indiana was dissolved.
Last week, a court appointed the Indianapolis Zoological Society to remove animals from the roadside zoo in Charlestown and care for them, a process that started Friday.
Owner Tim Stark claims he is being falsely represented by the media and People for the Ethical treatment of Animals.
People for the Ethical Treatment of Animals have filed a separate lawsuit involving the zoo's big cats, which is why they will remain on the premises for now.
Without the license, his zoo, Wildlife in Need, cannot legally host interactive animal shows on the property.
According to PETA, Stark sent the lions to Lowe's Greater Wynnewood Exotic Animal Park, which violated a previous ruling in the organization's case against Wildlife in Need.
The People for the Ethical Treatment of Animals (PETA) is asking a federal judge for an emergency hearing to question why a lion and tiger seemingly died at a controversial southern Indiana zoo.
PETA says that, for young people, "watching a nocturnal rodent being pulled from a fake hole isn't even worthy of a text message."