Officers performed life-saving measures until EMS arrived.
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LMPD's Homicide Unit is investigating, but have no suspects and have made no arrests.
Police believe the "involved parties" had a collision near Taylor Boulevard.
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KSP said the crash remains under investigation.
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Officers were called around 12:20 a.m. June 6 on a report of multiple people shot, LMPD spokesman John Bradley said at the time.
Louisville Metro Police arrested Dorian Lovett Friday, charging him with assault and wanton endangerment.
Melvin Carter, 39, was arrested Thursday on charges of assault and violation of an Emergency Protection Order.
LMPD is investigating the cause of the crash.
According to an arrest citation, Dashawn Garrison was driving erratically on South 26th Street when an officer began to follow him.
A 26-year-old man was charged with vehicular homicide in connection to a fatal crash in Nelson County last week, according to Kentucky State Police.